A fast, practical and reproducible procedure for the standardization of the cell density of an Aspergillus suspension

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Ricardo Araujo (Author) Other; rodrigues, ag (Author) FMUP; pina-vaz, c (Author) FMUP

Journal

Title: Journal of Medical Microbiology

Vol. 53

Pages: 783-786

ISSN: 0022-2615

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 32 Citations

Other information

Authenticus ID: P-000-97F

DOI: 10.1099/jmm.0.05425-0

Abstract (EN): The progressive increase of invasive disease and reports of resistance among Aspergillus species emphasizes the need for reproducible antifungal susceptibility testing. Inoculum standardization is a crucial step in such procedures. The objective of this study was to develop a fast and precise method of evaluating the cell density of an Aspergillus spore suspension, as an alternative to spectrometric readings or cell-counting with a haemocytometer. Densimat (bioMerieux) is a portable photometer that shows a good correlation with spectrometric readings and can advantageously replace the cumbersome, time-consuming method of cell-counting. Thus, Densimat brings significant improvement to the reproducibility and feasibility of standardization of a fungal inoculum.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4