Escherichia coli adhesion, biofilm development and antibiotic susceptibility on biomedical materials

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Luciana Gomes (Author) FEUP; Laura Silva (Author) FEUP; Manuel Simões (Author) FEUP; Luís Melo (Author) FEUP; Filipe Mergulhão (Author) FEUP

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 103 No. 1

Pages: 1414-1423

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 26 Citations

ISI Web of Science

Scopus - 50 Citations

Scientific classification

FOS: Medical and Health sciences

CORDIS: Technological sciences

Associated Projects

SYNBIOBACTHER - Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética

Filmstart - Estudo das condições iniciais que controlam a formação de biofilmes

Other information

Authenticus ID: P-00A-7EA

DOI: 10.1002/jbm.a.35277

Abstract (EN): The aim of this work was to test materials typically used in the construction of medical devices regarding their influence in the initial adhesion, biofilm development and antibiotic susceptibility of Escherichia coli biofilms. Adhesion and biofilm development was monitored in 12-well microtiter plates containing coupons of different biomedical materialssilicone (SIL), stainless steel (SS) and polyvinyl chloride (PVC)and glass (GLA) as control. The susceptibility of biofilms to ciprofloxacin and ampicillin was assessed, and the antibiotic effect in cell morphology was observed by scanning electron microscopy. The surface hydrophobicity of the bacterial strain and materials was also evaluated from contact angle measurements. Surface hydrophobicity was related with initial E. coli adhesion and subsequent biofilm development. Hydrophobic materials, such as SIL, SS, and PVC, showed higher bacterial colonization than the hydrophilic GLA. Silicone was the surface with the greatest number of adhered cells and the biofilms formed on this material were also less susceptible to both antibiotics. It was found that different antibiotics induced different levels of elongation on E. coli sessile cells. Results revealed that, by affecting the initial adhesion, the surface properties of a given material can modulate biofilm buildup and interfere with the outcome of antimicrobial therapy. These findings raise the possibility of fine-tuning surface properties as a strategy to reach higher therapeutic efficacy. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1414-1423, 2015.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10