Limited synergistic effect of antisense oligonucleotides against bcr-abl and transferrin receptor mRNA in leukemic cells in culture

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Vasconcelos, MH (Autor) Outra; Beleza, SS (Autor) Outra; Quirk, C (Autor) Outra; Maia, LF (Autor) Outra; Sambade, C (Autor) Outra; Guimaraes, JE (Autor) FMUP

Revista

Título: Cancer Letters

Vol. 152

Páginas: 135-143

ISSN: 0304-3835

Editora: Elsevier

Indexação

ISI Web of Knowledge - 24 Citações

Scopus - 26 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-001-048

DOI: 10.1016/s0304-3835(99)00441-3

Abstract (EN): The synergistic use of antisense oligonucleotides (ASOs) towards the bur-abl and the transferrin receptor (TfR) mRNA was studied in a chronic myeloid leukemia (CML) cell line, aiming to improve the efficiency of individual ASO treatment. At 20 muM concentration, bcr-abl ASOs reduced cell growth by 40% and was specific for cells that have the translocation: there was a 34% reduction of BCR-ABL protein. The TfR ASO reduced cell growth by 20% and decreased TfR protein by 24%. The ASOs were more potent at reducing cell growth when used in combination (respectively, -20 and -17% than bcr-abl ASO and TW ASO when used individually at the 10 muM concentration), thus we postulate that there is synergism of action. Cell cycle analysis also revealed that the sub-G1 peak was bigger in the synergistic treatment.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9