Rapid identification of Aspergillus fumigatus within the section Fumigati

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Rita Serrano (Autor) Outra; Leonor Gusmao (Autor) Outra; Antonio Amorim (Autor) FCUP; Ricardo Araujo (Autor) FCUP

Revista

Título: BMC Microbiology

Vol. 11

Página Final: 82

ISSN: 1471-2180

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 47 Citações

Scopus - 44 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-SBQ

DOI: 10.1186/1471-2180-11-82

Abstract (EN): Background: New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati. Misidentification of such fungal species, particularly of the human pathogens, Aspergillus lentulus, Neosartorya fischeri, Neosartorya hiratsukae, Neosartorya pseudofischeri and Neosartorya udagawae, has been increasingly reported by numerous clinical labs. Nevertheless, A. fumigatus still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of A. fumigatus to distinguish it from other species within the section Fumigati. Results: A multiplex PCR was developed using prior information based on beta-tubulin (beta tub) and rodlet A (rodA) partial gene sequences. PCR amplification of beta tub and rodA fragments resulted in a distinctive electrophoretic pattern in A. fumigatus and N. udagawae. The polymorphisms found in the smallest amplified sequence of beta tub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. beta tub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus A. lentulus. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish Aspergillus viridinutans, N. hiratsukae and N. udagawae. Conclusions: The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, A. fumigatus, in clinical laboratories.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7