In vitro hydroxyapatite adsorbed salivary proteins

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

vitorino, r (Autor) Outra; lobo, mjc (Autor) Outra; duarte, j (Autor) FADEUP; ferrer-correia, aj (Autor) Outra; tomer, kb (Autor) Outra; dubin, jr (Autor) Outra; domingues, pm (Autor) Outra; amado, fml (Autor) Outra

Revista

Título: Biochemical and Biophysical Research Communications

Vol. 320

Páginas: 342-346

ISSN: 0006-291X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 29 Citações

Scopus - 32 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-9DA

DOI: 10.1016/j.bbrc.2004.05.169

Abstract (EN): in spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5