A modular reactor to simulate biofilm development in orthopedic materials

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Joana Barros (Autor) FEUP; Liliana Grenho (Autor) Outra; Cândida M. Manuel (Autor) Outra; Carla Ferreira (Autor) FEUP; Luís F. Melo (Autor) FEUP; Olga C. Nunes (Autor) FEUP; Fernando J. Monteiro (Autor) FEUP; Maria P. Ferraz (Autor) Outra

Revista

Título: International Microbiology

Vol. 16 Nº 3

Páginas: 191-198

ISSN: 1139-6709

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Naturais

Projetos Associados

NaNOBiofilm - NaNOBiofilm - Superfícies de nanohidroxiapatite com caracteristicas antiobacterianas para prevenção de infecção óssea associada a biofilmes

Outras Informações

ID Authenticus: P-006-JWB

DOI: 10.2436/20.1501.01.193

Abstract (EN): Surfaces of medical implants are generally designed to encourage soft- and/or hard-tissue adherence, eventually leading to tissue- or osseo-integration. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. To understand the mechanisms of bone tissue infection associated with contaminated biomaterials, a detailed understanding of bacterial adhesion and subsequent biofilm formation on biomaterial surfaces is needed. In this study, a continuous-flow modular reactor composed of several modular units placed in parallel was designed to evaluate the activity of circulating bacterial suspensions and thus their predilection for biofilm formation during 72 h of incubation. Hydroxyapatite discs were placed in each modular unit and then removed at fixed times to quantify biofilm accumulation. Biofilm formation on each replicate of material, unchanged in structure, morphology, or cell density, was reproducibly observed. The modular reactor therefore proved to be a useful tool for following mature biofilm formation on different surfaces and under conditions similar to those prevailing near human-bone implants.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8