Use of fluorescence probes for detection of reactive nitrogen species: A review

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2006

Autores

Gomes, A (Autor) Outra; Fernandes, E (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: Journal of Fluorescence

Vol. 16

Páginas: 119-139

ISSN: 1053-0509

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 127 Citações

Scopus - 131 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-Q41

DOI: 10.1007/s10895-005-0030-3

Abstract (EN): The biological and toxicological effects that have been attributed to reactive nitrogen species (RNS) are increasingly stirring the scientific inquisitiveness about the molecular mechanisms involved. However, RNS present some characteristics that complicate their detection, namely their short lifetime and the normal presence of a variety of endogenous compounds capable of reacting with these reactive species, when the studies are performed in biological matrices. The development of methodologies capable of circumvent these difficulties is thus of fundamental importance. Fluorescence probes are particularly important due to their high sensibility and usefulness in temporal and spatial monitoring of RNS, particularly in microanalysis conditions in biological media akin to cells or tissues. In the present review is given an account of the fluorescence probes that have been used for detection of nitric oxide ((NO)-N-center dot), peroxynitrite anion (ONOO-), as well as of some of its derivatives in biological and nonbiological media.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 21