Optical probes for detection and quantification of neutrophils' oxidative burst. A review

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2009

Autores

Marisa Freitas (Autor) Outra; Jose L F C Lima (Autor) FFUP; Eduarda Fernandes (Autor) FFUP

Revista

Título: Analytica Chimica Acta

Vol. 649 Nº 1

Páginas: 8-23

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 106 Citações

Scopus - 123 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-FWR

DOI: 10.1016/j.aca.2009.06.063

Abstract (EN): Neutrophils. also known as polymorphonuclear leukocytes (PMN), are the most common type of white blood cells, comprising about 50-70% of all white blood cells. In the event of inflammatory processes, neutrophils display increased mobility, tissue influx ability, prolonged life span, and an increased phagocytic capacity, constituting the initial participants in the cellular defense of the organism. One of the most important defense systems of neutrophils corresponds to their ability to mediate a strong oxidative burst through the formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). While oxidative burst is important for the elimination of invading microorganisms, the overproduction of ROS and RNS or the impairment of endogenous antioxidant defenses may result to detrimental effects to the host. The nature and the extent of ROS and RNS production by neutrophils in response to different stimuli is, consequently, a matter of extensive research, with scientific reports showing an enormous variability on the detection methodologies employed. This review attempts to provide a critical assessment of the most common approaches to identify and quantify reactive species formed during the neutrophils' oxidative burst. The detection mechanisms and performance, as well as advantages and limitations of the different methodologies, are scrutinized, focusing on the use of fluorimetric, chemiluminometric and colorimetric probes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16