Xanthine oxidase inhibition by 1,3-dipropyl-8-sulfophenylxanthine (DPSPX), an antagonist of adenosine receptors

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Sousa, T (Autor) Outra; Morato, M (Autor) FFUP; Fernandes, E (Autor) FFUP; Carvalho, F (Autor) FFUP; Albino Teixeira, A (Autor) FMUP

Revista

Título: Journal of Enzyme Inhibition and Medicinal Chemistry

Vol. 19 Nº 1

Páginas: 11-15

ISSN: 1475-6366

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 5 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-BSC

DOI: 10.1080/14756360310001634910

Abstract (EN): Xanthine oxidase (XO), an enzyme involved in purine metabolism, is a source of either oxidants (superoxide radical) or antioxidants (uric acid). Interference with XO activity can lead to oxidative stress, thus contributing to the pathogenesis of cardiovascular diseases. The adenosine receptors antagonist, 1,3-dipropyl-8-sulfophenylxanthine (DPSPX), induces hypertension and cardiovascular injury in rats. Since DPSPX is a xanthine, we aimed at evaluating DPSPX's influence on XO activity to ascertain its contribution to DPSPX-induced hypertension. The activity of isolated XO in the presence of DPSPX was evaluated spectrophotometrically. Serum and urinary uric acid levels of DPSPX-treated rats were measured using a commercial kit. DPSPX inhibited XO activity in a concentration-dependent manner and reduced rat serum and urinary uric acid levels. It can be concluded that: DPSPX is an inhibitor of XO; decreased generation of uric acid may lead to oxidative stress, thus contributing to endothelial dysfunction and vascular morphological changes in DPSPX-treated rats.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5