An automatic Method to identify and extract information of DNA bands in Gel Electrophoresis Images

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2009

Autores

Caridade, CMR (Autor) Outra; Marcal, ARS (Autor) FCUP; Mendonca, T (Autor) FCUP; Pessoa, AM (Autor) Outra; Pereira, S (Autor) FCUP

Ata de Conferência Internacional

Título: 2009 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-20

Páginas: 1024-1027

Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society

Minneapolis, MN, SEP 03-06, 2009

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 2 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

Outras Informações

ID Authenticus: P-003-PRW

DOI: 10.1109/iembs.2009.5332462

Abstract (EN): This paper presents a system for the automatic processing of Digital Images obtained from Gel Electrophoresis. The system identifies automatically the number and the location of lanes in the digital image, as well as the location of bands on each lane, without any intervention from the user. A reference lane with a know substance is used to compute the molecular weight of the observed (unknown) bands. The system performance was tested using 12 images, obtained from 4 gels with 3 different exposures. A total of 5443 bands were tested in 12 images, 672 reference / observed lane pairs. The average error in the estimation of molecular weight of 9.2%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4