PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Laura Cerqueira (Autor) Outra; Ricardo M Fernandes (Autor) Outra; Rui M Ferreira (Autor) Outra; Fatima Carneiro (Autor) FMUP; Mario Dinis Ribeiro (Autor) FMUP; Ceu Figueiredo (Autor) FMUP; Charles W Keevil (Autor) Outra; Nuno F Azevedo (Autor) FEUP; Maria J Vieira (Autor) Outra

Revista

Título: BMC Microbiology

Vol. 11

Página Final: 101

ISSN: 1471-2180

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 35 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-RG9

DOI: 10.1186/1471-2180-11-101

Abstract (EN): Background: Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed. Results: The set of probes targeting the point mutations responsible for clarithromycin resistance was applied to H. pylori suspensions and showed 100% sensitivity and specificity (95% CI, 79.9-100 and 95% CI, 71.6-100 respectively). This method can also be amenable for application to gastric biopsy samples, as resistance to clarithromycin was also detected when histological slides were tested. Conclusions: The optimized PNA-FISH based diagnostic method to detect H. pylori clarithromycin resistance shown to be a very sensitive and specific method for the detection of clarithromycin resistance in the H. pylori smears and also proved to be a reliable method for the diagnosis of this pathogen in clinical samples and an alternative to existing plating methods.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7