Enzymatic Determination of Glucose in Milk Samples by Sequential Injection Analysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Alberto N Araujo (Autor) FFUP; Jose L F C Lima (Autor) FFUP; Paula C A G Pinto (Autor) FFUP; Lucia L M F S Saraiva (Autor) FFUP

Revista

Título: Analytical Sciences

Vol. 25 Nº 5

Páginas: 687-692

ISSN: 0910-6340

Editora: Japan Society for Analytical Chemistry

Indexação

ISI Web of Knowledge - 5 Citações

ISI Web of Science

Scopus - 6 Citações

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-K69

DOI: 10.2116/analsci.25.687

Resumo (PT): The present work describes a comparative study involving two sequential injection enzymatic procedures for the spectrophotometric determination of glucose in milk samples. The determinations were based on the use of the enzymes, glucose oxidase and peroxidase, in solution or immobilized, and 4-aminophenazone and phenol as chromogenic reagents. In the first procedure, 8.4 IU of glucose oxidase and 0.5 IU of peroxidase were consumed in each determination. In the second procedure, 107 IU of glucose oxidase and 105 IU of peroxidase, immobilized in glass beads, were sequentially packed into the same reactor. The reactor allowed us to perform about 600 continuous determinations with no activity loss. The proposed systems allowed the determination of glucose up to 120 mg L-1 in milk samples with a precision, considering the RSD (%) values, lower than 3.5%. The results obtained for the two systems were comparatively evaluated against a routine enzymatic method, showing a relative deviation of less than 3%. <br> <br> <a target="_blank" href="http://www.jstage.jst.go.jp/article/analsci/25/5/25_687/_article"> Texto integral</a> <br> <br>

Abstract (EN): The present work describes a comparative Study involving two Sequential injection enzymatic procedures for the spectrophotometric determination of glucose in milk Samples. The determinations were based oil the use of the enzymes, glucose oxidase and peroxidase, in solution or immobilized, and 4-aminophenazone and phenol as chromogenic reagents. In the first procedure, 8.4 IU of glucose oxidase and 0.5 IU of peroxidase were consumed in each determination. In the second procedure, 107 IU of glucose oxidase and 105 IU of peroxidase, immobilized in glass heads, Were sequentially packed into the same reactor. The reactor allowed LIS to perform about 600 continuous determinations with no activity loss. The proposed systems allowed the determination of glucose up to 120 mg L(-1) in milk samples with it precision, considering the RSD (%) values, lower than 3.5%. The results obtained for the two systems were Comparatively evaluated against it routine enzymatic method, showing it relative deviation of less than 3%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6