Development and Validation of a Matrix Solid-Phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Cristina M D Dias Soares (Autor) Outra; Rita C Alves (Autor) Outra; Susana Casal (Autor) FFUP; Beatriz B P P Oliveira (Autor) FFUP; Jose Oliveira Fernandes (Autor) FFUP

Revista

Título: Journal of Food Science

Vol. 75

Páginas: T57-T63

ISSN: 0022-1147

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 35 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-003-7X6

DOI: 10.1111/j.1750-3841.2010.01545.x

Abstract (EN): The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C(18) sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C(18)/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 mu g/ kg and a LOQ of 10 mu g/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7