Uptake studies in rat Peyer's patches, cytotoxicity and release studies of alginate coated chitosan nanoparticles for mucosal vaccination

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Olga Borges (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP; Stefan G Romeijn (Autor) Outra; Maryam Amidi (Autor) Outra; Adriano de Sousa (Autor) Outra; Gerrit Borchard (Autor) Outra; Hans E Junginger (Autor) Outra

Revista

Título: Journal of Controlled Release

Vol. 114 Nº 3

Páginas: 348-358

ISSN: 0168-3659

Editora: Elsevier

Indexação

ISI Web of Knowledge - 121 Citações

ISI Web of Science

Scopus - 155 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-004-H86

DOI: 10.1016/j.jconrel.2006.06.011

Resumo (PT): The design of particulate vaccine delivery systems, particularly for mucosal surfaces, has been a focus of interest in recent years. In this context, we have previously described the development and the characterization of a new nanosized delivery system, consisting of a model antigen adsorbed to chitosan particles and coated with sodium alginate. In the present work the ovalbumin release profiles from these coated nanoparticles in different pH buffers were investigated and compared to those of the uncoated particles. Cytotoxicity of the polymers and nanoparticles was assessed using the MTT assay. Finally, particle uptake studies in rat Peyer's patches were performed. It was demonstrated that the coating of the nanoparticles with sodium alginate not only avoided a burst release observed with uncoated particles but also increased the stability of the particles at pH 6.8 and 7.4 at 37 °C. At neutral pH, the release was lower than 5% after 3.5 h incubation in a low ionic strength buffer. For both, chitosan and alginate polymers, and for the nanoparticles, comparable cell viability data close to 100%, were obtained. Additionally, based on confocal laser scanning microscopy observations, it was shown that alginate coated nanoparticles were able to be taken up by rat Peyer's patches, rendering them suitable carriers for intestinal mucosal vaccination. <br> <br> Keywords: Coated nanoparticles; Chitosan; Sodium alginate; Peyer's patches; Cytotoxicity <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3D-4K6CHRP-1&_user=2460038&_coverDate=09%2F12%2F2006&_rdoc=10&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234944%232006%23998859996%23631860%23FLA%23display%23Volume)&_cdi=4944&_sort=d&_docanchor=&_ct=17&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=bdbbc34388c32fd8568bb691d38bd762"> Texto integral</a> <br> <br>

Abstract (EN): The design of particulate vaccine delivery systems, particularly for mucosal surfaces, has been a focus of interest in recent years. In this context, we have previously described the development and the characterization of a new nanosized delivery system, consisting of a model antigen adsorbed to chitosan particles and coated with sodium alginate. In the present work the ovalbumin release profiles from these coated nanoparticles in different pH buffers were investigated and compared to those of the uncoated particles. Cytotoxicity of the polymers and nanoparticles was assessed using the MTT assay. Finally, particle uptake studies in rat Peyer's patches were performed. It was demonstrated that the coating of the nanoparticles with sodium alginate not only avoided a burst release observed with uncoated particles but also increased the stability of the particles at pH 6.8 and 7.4 at 37 degrees C. At neutral pH, the release was lower than 5% after 3.5 h incubation in a low ionic strength buffer. For both, chitosan and alginate polymers, and for the nanoparticles, comparable cell viability data close to 100%, were obtained. Additionally, based on confocal laser scanning microscopy observations, it was shown that alginate coated nanoparticles were able to be taken up by rat Peyer's patches, rendering them suitable carriers for intestinal mucosal vaccination.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11