Simultaneous determination of carbonyl compounds related to thermal treatment and oxidative stability of infant formulas by gas-diffusion microextraction and high-performance liquid chromatography with ultraviolet detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

Custodio-Mendoza, JA (Autor) Outra; Muñoz-Menendez, L (Autor) Outra; España-Fariñas, MP (Autor) Outra; Valente, IM (Autor) FCUP; Rodrigues, JA (Autor) FCUP; Almeida, PJ (Autor) Outra; Lorenzo, RA (Autor) Outra; Carro, AM (Autor) Outra

Revista

Título: Analytica Chimica Acta

Vol. 1288

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-RGT

DOI: 10.1016/j.aca.2023.342164

Abstract (EN): Infant formulae are the only possible alternative to breastfeeding during the first year of life, so it is crucial to assure their innocuousness. Infant formula undergoes heat treatments to ensure safety and shelf life. However, such processes impact health as they lead to the formation of malondialdehyde, acrolein, and alpha-dicarbonyl compounds, related to Maillard reaction. Thus, there is a need for improved analytical methods to ensure the safety, quality, and nutritional value of infant formulae, and also exploring the potential of specific compounds as indicators for quality control and monitoring purposes. We developed and validated a novel, efficient, and cost-effective method using gas -diffusion microextraction for the simultaneous quantification of carbonyl compounds in infant formula. Malondialdehyde, acrolein, glyoxal, methylglyoxal, and diacetyl were detected as o-phenylenediamine derivatives using HPLC with UV detection. Parameters influencing extraction efficiency were studied using an asymmetric screening design. The validated method has shown excellent linearity, sensitivity, accuracy, and precision. It was applied to analyze 26 infant formula samples, including starter, follow-up, and special formulated powdered infant formula. Methylglyoxal was found in all samples (0.201-3.153 mu g mL(-1)), while malondialdehyde was present only in certain starter formulas (1.033-1.802 mu g mL(-1)). Acrolein (0.510-3.246 mu g mL(-1)), glyoxal (0.109-1.253 mu g mL(-1)), and diacetyl (0.119-2.001 mu g mL(-1)) were detected in various sample types. Principal components and hierarchical cluster analyses have showcased distinct sample clustering based on analyte contents. This study presents a novel methodology for the analysis of markers of thermal treatment and oxidative stability in infant formula. It contributes to the characterization of the products' composition and quality control of infant formulae, thereby enhancing their safety and nutritional adequacy. This study also presents the first reported quantification of acrolein in infant formula and introduces the application of the acrolein-o-phenyl- enediamine derivative for food analysis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11