Plasmonic genosensor for detecting hazelnut Cor a 14-encoding gene for food allergen monitoring

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Moreira P. (Autor) Outra; Costa J. (Autor) Outra; Villa C. (Autor) Outra; Mafra I. (Autor) Outra; Brandão A.T.S.C. (Autor) Outra; Dias C. (Autor) Outra; Silva, F (Autor) FCUP; Carlos M Pereira (Autor) FCUP; Costa R. (Autor) FCUP

Revista

Título: Analytica Chimica Acta

Vol. 1259

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00Y-7RZ

DOI: 10.1016/j.aca.2023.341168

Abstract (EN): A plasmonic nanostructure was constructed as a biorecognition element coupled to an optical sensing platform in sandwich format, targeting the hazelnut Cor a 14 allergen-encoding gene. The analytical performance of the genosensor presented a linear dynamic range between 100 amol L-1 and 1 nmol L-1 , a limit of detection (LOD) < 19.9 amol L-1 , and a sensitivity of 13.4 +/- 0.6 m.. The genosensor was successfully hybridized with hazelnut PCR products, tested with model foods, and further validated by real-time PCR. It reached a LOD <0.001% (10 mg kg(-1) ) of hazelnut in wheat material (corresponding to 1.6 mg kg(-1) of protein) and a sensitivity of 17.2 +/- 0.5 m. for a linear range of 0.001%-1%. Herein, a new genosensing approach is proposed as a highly sensitive and specific alternative tool with potential application in monitoring hazelnut as an allergenic food, protecting the health of sensitized/allergic individuals.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11