Molinate biodegradation in soils: natural attenuation versus bioaugmentation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Ana Rita Lopes (Autor) FEUP; Anthony S. Danko (Autor) FEUP; Célia M. Manaia (Autor) Outra; Olga C. Nunes (Autor) FEUP

Revista

Título: Applied Microbiology and Biotechnology

Vol. 97 Nº 6

Páginas: 2691-2700

ISSN: 0175-7598

Editora: Springer Nature

Indexação

ISI Proceedings

ISI Web of Knowledge - 10 Citações

Scopus - 15 Citações

Classificação Científica

CORDIS: Ciências Naturais

FOS: Ciências exactas e naturais > Ciências biológicas

Projetos Associados

POCI/AMB/59836/2004 - Biorremediação de solos e águas contaminadas com pesticidas

PTDC/AMB/66653/2006 - Caracterização de uma nova via metabólica: mineralização do herbicida molinato

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Outras Informações

ID Authenticus: P-002-020

DOI: 10.1007/s00253-012-4096-y

Abstract (EN): The aims of the present study were to assess the potential of natural attenuation or bioaugmentation to reduce soil molinate contamination in paddy field soils and the impact of these bioremediation strategies on the composition of soil indigenous microbiota. A molinate mineralizing culture (mixed culture DC) was used as inoculum in the bioaugmentation assays. Significantly higher removal of molinate was observed in bioaugmentation than in natural attenuation microcosms (63 and 39 %, respectively) after 42 days of incubation at 22 A degrees C. In the bioaugmentation assays, the impact of Gulosibacter molinativorax ON4(T) on molinate depletion was observed since the gene encoding the enzyme responsible for the initial molinate breakdown (harboured by that actinobacterium) was only detected in inoculated microcosms. Nevertheless, the exogenous mixed culture DC did not overgrow as the heterotrophic counts of the bioaugmentation microcosms were not significantly different from those of natural attenuation and controls. Moreover, the actinobacterial clone libraries generated from the bioaugmentation microcosms did not include any 16S rRNA gene sequences with significant similarity to that of G. molinativorax ON4(T). The multivariate analysis of the 16S rRNA DGGE patterns of the soil microcosm suggested that the activity of mixed culture DC did not affect the soil bacterial community structure since the DGGE patterns of the bioaugmentation microcosms clustered with those of natural attenuation and controls. Although both bioremediation approaches removed molinate without indigenous microbiota perturbation, the results suggested that bioaugmentation with mixed culture DC was more effective to treat soils contaminated with molinate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10