Diversity and antibiotic resistance patterns of Sphingomonadaceae isolates from drinking water

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Ivone Vaz Moreira (Autor) FEUP; Olga C. Nunes (Autor) FEUP; Célia M. Manaia (Autor) Outra

Revista

Título: Applied and Environmental Microbiology

Vol. 77 Nº 16

Páginas: 5697-5706

ISSN: 0099-2240

Editora: American Society for Microbiology

Indexação

ISI Web of Knowledge - 81 Citações

ISI Web of Science

Scopus - 117 Citações

COMPENDEX

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

CORDIS: Ciências Naturais > Ciências biológicas > Biologia > Microbiologia ; Ciências Tecnológicas > Engenharia > Engenharia química

Outras Informações

ID Authenticus: P-002-PAD

DOI: 10.1128/aem.00579-11

Abstract (EN): Sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the genera Sphingomonas (n = 27), Sphingobium (n = 28), Novosphingobium (n = 12), Sphingopyxis (n = 7), and Blastomonas (n = 12). The patterns of susceptibility to five classes of antibiotics were analyzed and compared for the different sites of isolation and taxonomic groups. Colistin resistance was observed to be intrinsic (92%). The highest antibiotic resistance prevalence values were observed in members of the genera Sphingomonas and Sphingobium and for beta-lactams, ciprofloxacin, and cotrimoxazole. In tap water and in water from dental chairs, antibiotic resistance was more prevalent than in the other samples, mainly due to the predominance of isolates of the genera Sphingomonas and Sphingobium. These two genera presented distinct patterns of association with antibiotic resistance, suggesting different paths of resistance development. Antibiotic resistance patterns were often related to the species rather than to the site or strain, suggesting the importance of vertical resistance transmission in these bacteria. This is the first study demonstrating that members of the family Sphingomonadaceae are potential reservoirs of antibiotic resistance in drinking water.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10