Sprouting Angiogenesis: A Numerical Approach with Experimental Validation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Guerra, A (Autor) Outra; Belinha, J (Autor) Outra; Mangir, N (Autor) Outra; MacNeil, S (Autor) Outra; Renato Natal Jorge (Autor) FEUP

Revista

Título: Annals of Biomedical Engineering

ISSN: 0090-6964

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-00S-RJA

DOI: 10.1007/s10439-020-02622-w

Abstract (EN): A functional vascular network is essential to the correct wound healing. In sprouting angiogenesis, vascular endothelial growth factor (VEGF) regulates the formation of new capillaries from pre-existing vessels. This is a very complex process and mathematical formulation permits to study angiogenesis using less time-consuming, reproducible and cheaper methodologies. This study aimed to mimic the chemoattractant effect of VEGF in stimulating sprouting angiogenesis. We developed a numerical model in which endothelial cells migrate according to a diffusion-reaction equation for VEGF. A chick chorioallantoic membrane (CAM) bioassay was used to obtain some important parameters to implement in the model and also to validate the numerical results. We verified that endothelial cells migrate following the highest VEGF concentration. We compared the parameters-total branching number, total vessel length and branching angle-that were obtained in thein silicoand thein vivomethodologies and similar results were achieved (p-valuesmaller than 0.5;n= 6). For the difference between the total capillary volume fractions assessed using both methodologies values smaller than 15% were obtained. In this study we simulated, for the first time, the capillary network obtained during the CAM assay with a realistic morphology and structure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14