Ultrasonic protein crystallization: Promoting nucleation in microdroplets through pulsed sonication

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Ferreira, J (Autor) FEUP; Opsteyn, J (Autor) Outra; Fernando Rocha (Autor) FEUP; F. Castro (Autor) FEUP; Kuhn, S (Autor) Outra

Revista

Título: Chemical Engineering Research and Design

Vol. 162

Páginas: 249-257

ISSN: 0263-8762

Editora: Institute of Chemical Engineers

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-00S-NJJ

DOI: 10.1016/j.cherd.2020.08.014

Abstract (EN): Droplet microfluidics allows a higher degree of control over the crystallization conditions than conventional methodologies. To extend this approach, this work explores the synergistic effect of low-frequency pulsed ultrasound on lysozyme crystallization in microdroplets. Pulsed actuation allows control of the crystallization temperature, while the ultrasound effect significantly reduces the induction time and crystal size. Therefore, protein nucleation is enhanced by pulsed sonication without causing precipitation, resulting in uniform crystal size. Finally, the initial supersaturation ratio has a crucial contribution to the crystal size for silent experiments, while a threshold for induction time is observed in ultrasonic crystallization.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9