Ofloxacin Determination in Urine, Serum and Pharmaceuticals Using an Automatic Flow Potentiometric System

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Pimenta, AM (Autor) Outra; Souto, MRS (Autor) Outra; Catarino, RIL (Autor) Outra; Leal, MFC (Autor) Outra; Lima, JLFC (Autor) FFUP

Revista

Título: Analytical Sciences

Vol. 29

Páginas: 893-898

ISSN: 0910-6340

Editora: Japan Society for Analytical Chemistry

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-008-ENK

DOI: 10.2116/analsci.29.893

Abstract (EN): An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 x 10(-5) M, a linear response over the concentrations of 2 x 10(-5) to 5 x 10(-3) M (slope of 57.4 mV decade(-1)) and a wide working pH range (2.1 - 6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6