Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Pinto, G (Autor) Outra; Valentim, H (Autor) Outra; Costa, A (Autor) Outra; Castro, S (Autor) Outra; Santos, C (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Vol. 38

Páginas: 569-572

ISSN: 1054-5476

Editora: Springer

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 35 Citações

Outras Informações

ID Authenticus: P-000-MHM

DOI: 10.1079/ivp2002352

Abstract (EN): Somatic embryos were obtained from a 60-yr-old Quercus suber L. tree. Leaf explants were cultivated on Murashige and Skoog medium with 30 gl(-1) sucrose, 3 gl(-1) gelrite, pH adjusted to 5.8, and different growth regulator combinations. Callus induction took place at 24 +/- 1degreesC in the dark during the first 3 wk. After 3 mo. calluses that showed embryogenic structures were transferred to the same medium without growth regulators. Somatic embryogenesis was only observed in calluses induced on E3 medium (supplemented with 4.5 muM 2,4-dichlorophenoxyacetic acid and 9.0 muM zeatin). On average, 7.5% of the initial explants formed embryogenic calluses in this medium. Somatic embryo proliferation was high due to secondary embryogenesis. On average, 10% of the somatic embryos germinated and 40% of these germinated embryos converted into plants. Plants were elongated on the same medium without growth regulators and acclimated to greenhouse conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4