A FIA-enzymatic method for the determination of L(+) lactate: application in parenteral solution analysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Barrado, E (Autor) Outra; Vela, MH (Autor) Outra; Lapa, RAS (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: QUIMICA ANALITICA

Vol. 19

Páginas: 241-246

ISSN: 0212-0569

Editora: Springer

Indexação

ISI Web of Knowledge - 1 Citação

Outras Informações

ID Authenticus: P-001-25P

Abstract (EN): A flow injection system for the determination of L(+)lactate in parenteral solutions was developed. The system is based on the amperometric detection of hydrogen peroxide, produced when lactate is converted to pyruvate by the enzyme lactate oxidase over a platinum electrode. The flow injection manifold contains a dialysis unit to maintain the enzyme in a closed re-circulation loop. Determinations are made without the need to pre-treat the sample. Under optimal conditions of pH, temperature and hydrodynamic and geometric variables obtained by means of a L-9(3(4)) Taguchi parameter design, a linear response was achieved for lactate concentrations within the range 2 to 30 mM, working at 37 degreesC. Reproducibility was good (RSD < 5%, n = 20) at a sampling rate of 25 determinations/hour. The results obtained were in close agreement with those of a reference method (European Pharmacopeia).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6