Effect of solvent system on extractability of lipidic components of Scenedesmus obliquus (M2-1) and Gloeothece sp. on antioxidant scavenging capacity thereof

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

F. Xavier Malcata (Autor) FEUP; Helena M. Amaro (Autor) Outra; Fátima Fernandes (Autor) Outra; Patrícia Valentão (Autor) Outra; Paula B Andrade (Autor) FFUP; Isabel Sousa Pinto (Autor) FCUP; A. Catarina Guedes (Autor) Outra

Revista

Título: Marine Drugs

Nº de Série 1660-3397 Vol. 13 Nº 10

Páginas: 6453-6471

ISSN: 1660-3397

Editora: MDPI

Indexação

ISI Web of Knowledge - 45 Citações

ISI Web of Science

Scopus - 54 Citações

Classificação Científica

CORDIS: Ciências Tecnológicas

FOS: Ciências da engenharia e tecnologias

Projetos Associados

UI0511 - Incentivo/EQB/UI0511/2014

Outras Informações

ID Authenticus: P-00G-TBD

DOI: 10.3390/md13106453

Assunto: carotenoid; PUFA; extract; microalga; cyanobacteria; ABTS+¿; DPPH¿; superoxide (O2¿¿) assay; nitric oxide (¿NO-) assay

Abstract (EN): Microalgae are well known for their biotechnological potential, namely with regard to bioactive lipidic components¿especially carotenoids and polyunsaturated fatty acids (PUFA), well-known for therapeutic applications based on their antioxidant capacity. The aim of this work was to evaluate the influence of four distinct food-grade solvents upon extractability of specific lipidic components, and on the antioxidant capacity exhibited against both synthetic (2,2-diphenyl-1-picrylhydrazyl (DPPH¿) and 2,2¿-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+¿)) and biological reactive species (O2¿- and ¿NO-). A eukaryotic microalga (Scenedesmus obliquus (M2-1)) and a prokaryotic one (Gloeothece sp.) were used as case studies. Concerning total antioxidant capacity, the hexane:isopropanol (3:2) and acetone extracts of Sc. obliquus (M2-1) were the most effective against DPPH¿ and ABTS+¿, respectively. Gloeothece sp. ethanol extracts were the most interesting scavengers of O2¿-, probably due the high content of linolenic acid. On the other hand, acetone and hexane:isopropanol (3:2) extracts were the most interesting ones in ¿NO- assay. Acetone extract exhibited the best results for the ABTS assay, likely associated to its content of carotenoids, in both microalgae. Otherwise, ethanol stood out in PUFA extraction. Therefore, profiles of lipidic components extracted are critical for evaluating the antioxidant performance¿which appears to hinge, in particular, on the balance between carotenoids and PUFAs.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 19