Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Sonia Soares (Autor) Outra; Joana S Amaral (Autor) Outra; Beatriz B P P Oliveira (Autor) FFUP; Isabel Mafra (Autor) Outra

Revista

Título: Meat Science

Vol. 98

Páginas: 41-46

ISSN: 0309-1740

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 20 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-009-HKN

DOI: 10.1016/j.meatsci.2014.04.002

Abstract (EN): In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6