Direct Identification and Characterization of Phenolic Compounds from Crude Extracts of Buds and Internodes of Grapevine (Vitis vinifera cv Merlot)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Said Qsaib (Autor) Outra; Nuno Mateus (Autor) FCUP; Fatima Ez zohra Ikbarl (Autor) Outra; Lala Aicha Rifai (Autor) Outra; Victor de Freitas (Autor) Outra; Tayeb Koussa (Autor) Outra

Revista

Título: Natural product communications

Vol. 9

Páginas: 1569-1572

ISSN: 1934-578X

Editora: SAGE

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-009-YTP

Abstract (EN): The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and epsilon-viniferin had similar levels in buds and intemodes while six other compounds identified had higher levels in buds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica