Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Raquel R Maia (Autor) Outra; Mariana Barbosa (Autor) Outra; David B Gomes (Autor) Outra; Nuno Vale (Autor) FCUP; Paula Gomes (Autor) FCUP; Pedro L Granja (Autor) FEUP; Cristina C Barrias (Autor) Outra

Revista

Título: Journal of Controlled Release

Vol. 189

Páginas: 158-168

ISSN: 0168-3659

Editora: Elsevier

Indexação

ISI Web of Knowledge - 52 Citações

Scopus - 58 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-009-PHN

DOI: 10.1016/j.jconrel.2014.06.030

Abstract (EN): The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11