Hyaluronic acid/chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Hanna Hartmann (Autor) Outra; Susanne Hossfeld (Autor) Outra; Burkhard Schlosshauer (Autor) Outra; Ursula Mittnacht (Autor) Outra; Ana Paula Pego (Autor) FEUP; Martin Dauner (Autor) Outra; Michael Doser (Autor) Outra; Dieter Stoll (Autor) Outra; Rumen Krastev (Autor) Outra

Revista

Título: Journal of Controlled Release

Vol. 168

Páginas: 289-297

ISSN: 0168-3659

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 8 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-ZZ8

DOI: 10.1016/j.jconrel.2013.03.026

Abstract (EN): Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/ siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multilayers (PEMs) composed of hyaluronic acid and chitosan for sustained and localized drug delivery. Regular PEM build-up, successful integration of NPs and controlled release under physiological conditions were shown. Biological efficacy was evaluated in neuronal cell culture concerning cell adhesion, viability, NPs uptake and gene silencing. The additionally shown biological functionalization of neuronal implants possesses potential for future applications in the field of regenerative medicine and treatment of spinal cord injuries. (C) 2013 Elsevier B.V. All rights reserved.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9