Specific detection of Pneumocystis jirovecii in clinical samples by flow cytometry

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

barbosa, j (Autor) Outra; costa-de-oliveira, s (Autor) FMUP; silva, at (Autor) Outra; rodrigues, ag (Autor) FMUP; pina-vaz, c (Autor) FMUP

Revista

Título: Methods in molecular biology (Clifton, N.J.)

Vol. 968

Páginas: 203-211

ISSN: 1064-3745

Editora: Springer Nature

Indexação

ISI Web of Knowledge

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-008-PNP

DOI: 10.1007/978-1-62703-257-5-16

Abstract (EN): Pneumocystis jirovecii is an opportunistic pathogen responsible for severe pneumonia in immunocompromised patients. Flow cytometry (FC) is a method widely used in different areas of clinical diagnosis like hematology and immunology. Recently it has started to be used in microbiology with a great potential for diagnosis of emergent microorganisms in clinical samples, especially when present in low numbers. The detection of Pneumocystis jirovecii in respiratory samples can be performed by FC, using speci fic monoclonal antibodies. Considering clinical diagnosis as a reference method, we previously showed FC to be 100% sensitive and speci fic when compared to immuno fl uorescence. Being an automated method, it is faster and less subject to human error than microscopic evaluation. © Springer Science+Business Media New York 2013.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9