| Resultados: |
Os principais outputs do projeto foram:
Papers in international journals
• Soares A, Azevedo A, Gomes LC, Mergulhão FJ. Recombinant protein expression in biofilms. AIMS Microbiol. 2019 27;5(3):232-250. doi: 10.3934/microbiol.2019.3.232.
• Gomes L, Monteiro G, Mergulhão F. The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms. Int J Mol Sci. 2020 16;21(2):576. doi: 10.3390/ijms21020576.
• Soares A, Gomes LC, Monteiro GA, Mergulhão FJ. The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression. Appl. Sci. 2021; 11(18):8667. doi: 10.3390/app11188667.
• Gomes LC, Mergulhão FJM. A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions. Microorganisms 2021; 9(9):1993. doi: 10.3390/microorganisms9091993.
• Gomes LC, Ferreira C, Mergulhão FJ. Implementation of a Practical Teaching Course on Protein Engineering. Biology 2022; 11(3):387. https://doi.org/10.3390/biology11030387
• Soares A, Gomes LC, Monteiro GA, Mergulhão FJ. Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression. Microorganisms 2022; 10(5):931. https://doi.org/10.3390/microorganisms10050931
• Carvalho FM, Azevedo A, Ferreira MM, Gomes LC, Mergulhão FJ. Advances on Bacterial and Fungal Biofilms for the production of value-added compounds. (In preparation)
• Azevedo A, Carvalho FM, Gomes LC, Monteiro GA, Mergulhão FJ. Impact of surface material and nutrient conditions on Green Fluorescent Protein (GFP) production in Escherichia coli biofilms. Biology. (Submitted)
• Soares, A, Carvalho, FM, Gomes, LC, Monteiro GA, Mergulhão FJ. Effect of IPTG induction on specific GFP production by E. coli biofilms. (In preparation)
• Azevedo, A., Carvalho FM, Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Production of curcumin in biofilms developed under controlled flow conditions. (In preparation)
Book chapters
• Luciana Gomes and Filipe José Mergulhão. "Production of Recombinant Proteins in Escherichia coli Biofilms: Challenges and Opportunities". In Advances in Medicine and Biology, 183-217. Nova Science Publishers, Inc., 2019.
• Azevedo, A., F.M. Carvalho, L.C. Gomes, and F.J. Mergulhão. “Use of Bacterial Biofilms to Produce High Added-Value Compounds”, in Understanding microbial biofilms: Fundamentals to applications, S. Das and N. Kungwani, Editors. 2022, Academic Press. ISBN: 9780323999779.
Communications in international meetings
• Gomes, L.C., Alves, P., Soares, A., Mergulhão, F.J. Microscopy investigation of bacterial biofilm development in flow systems. INTERM 2019 — 6th International Symposium on Microscopy and Spectroscopy, May 2019, Turkey (oral presentation).
• Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Recombinant Protein Production and Plasmid Stability in Escherichia coli Biofilms. Biofilms 9, 2020. doi: 10.5194/biofilms9-65 (oral presentation, online).
• Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Surface and nutrient media: impact on E. coli biofilms expressing Green Fluorescent Protein (GFP). Biointerfaces International Zurich 2021, August 2021, (poster presentation, online). http://www.biointerfaces.ch/international/2021/
• Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Heterologous protein expression in E. coli biofilms: gene dosage and induction effects. Biointerfaces International Zurich 2021, August 2021 (poster presentation, online). |
| Observações: |
continuação dos outputs (apresentações orais em conferências internacionais)
• Gomes, L.C., Monteiro, G.A., Mergulhão, F.J. Biofilm systems as tools in the production of recombinant proteins and other added-value compounds. 3rd Aachen Protein Engineering Symposium, September 2021 (flash presentation, online).
• Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Expression of Green Fluorescent Protein (GFP) in Escherichia coli biofilms: impact of surface and nutrient conditions. International Biodeterioration & Biodegradation Symposium (IBBS18), September 2021 (poster presentation, online). https://www.ibbs18.org/
• Soares, A., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Effect of nutrient medium composition on heterologous protein production in biofilms. International Biodeterioration & Biodegradation Symposium (IBBS18), September 2021 (poster presentation, online). https://www.ibbs18.org/
• Soares, A., Gomes, L.C., Mergulhão F.J. Using microscopy to assess the effect of medium composition during recombinant protein production in biofilms. INTERM 2022 — 9th International Symposium on Microscopy and Spectroscopy, April 2022, Turkey (oral presentation).
• Carvalho, F.M., Azevedo, A., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Expression of recombinant Green Fluorescent Protein (GFP) using Escherichia coli biofilms: effects of culture media and surface properties. Biofilms 10, May 2022 (poster presentation, online). https://www.ufz.de/index.php?en=49335
• Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Green Fluorescent Protein (GFP) expression in Escherichia coli biofilms: effects of surface and nutrient conditions. SurfSAFE Scientific Workshop 1: Characterization of non-biotoxic antimicrobial, anti-adhesive and biomimetic surfaces, June 2021 (oral presentation, online).
• Azevedo, A., Carvalho, F.M., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. The influence of surface and media composition on the expression of Green Fluorescent Protein (GFP) in Escherichia coli biofilms. SurfSAFE Scientific Workshop 2: Protein adsorption and biofilm formation, December 2021, Netherlands (oral presentation).
Communications in national meetings
• Soares, A., Gomes, L.C., Mergulhão F.J. Effect of nutrient medium composition on the expression of a recombinant protein in Escherichia coli biofilms". Doctoral Congress in Engineering 2019 - DCE 2019, 2019, FEUP, Portugal (poster presentation).
https://paginas.fe.up.pt/~dce/2019/programme/poster-session/.
• Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A., Mergulhão F.J., Optimization of enhanced Green Fluorescent Protein (eGFP) production in Escherichia coli biofilms. DCE 21 - 4th Doctoral Congress in Engineering, June 2021 (poster presentation, online). https://paginas.fe.up.pt/~dce/2021/.
Organization of seminars and conferences
• Scientific Workshop 1 – “Characterization of non-biotoxic, antimicrobial, anti-adhesive and biomimetic surfaces”, June 2021 (online).
• Scientific Workshop 2 – “Protein adsorption and biofilm formation”, December 2021, Groningen, Netherlands.
• Training School 1 – “Evaluation of the surface performance”, March 2022, Porto, Portugal.
PhD thesis
• Alexandra Soares, “Optimizing Recombinant Protein Production in Escherichia coli Biofilms” (FCT, Ref. SFRH/141614/2018) (end in September 2022)
• Ana Azevedo, “The use of Escherichia coli biofilms for the production of recombinant proteins and other added-value compounds” |