Sequential Injection Analysis of Ampicillin in Pharmaceuticals by Using Potentiometric Detectors Based on PVC and Sol-Gel Membranes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Nestor Zárate (Autor) Outra; Alberto N. Araújo (Autor) FFUP; M. Conceiçao B. S. M. Montenegro (Autor) FFUP; Ricardo Pérez-Olmos (Autor) Outra

Revista

Título: American Journal of Analytical Chemistry

Vol. 2 Nº 4

Páginas: 491-499

ISSN: 2156-8278

Editora: Scientific Research

Classificação Científica

FOS: Ciências médicas e da saúde > Ciências da saúde

CORDIS: Ciências Físicas > Química > Química analítica

Outras Informações

Resumo (PT): This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, in-corporated in both PVC and sol-gel membranes and directly applied onto a conductive graphite/epoxy resin support. The units were constructed without inner reference solution adopting conventional configuration and in the case of PVC membrane the tubular configuration was also adopted. The good working character-istics of these electrodes, made possible its application to the determination of ampicillin in pharmaceuticals formulations, both in conventional batch analysis and in flow conditions, when the electrodes were coupled to a SIA system. In the last case the potentiometric sensors presented linear response towards ampicillin concentration between 5.0 × 10–4 and 5.0 × 10–2 mol·l–1 with slopes of –57.4 and –63.5 mV·dec–1 for the PVC and sol-gel membranes, respectively. The developed procedures enable mean relative standard deviations better than 3% for all the samples analysed. The obtained results do not statistically differ from those fur-nished by applying the HPLC reference method. <br> <br> Keywords: Ampicillin, SIA, Potentiometric Detection, Pharmaceutical Formulations <br> <a target="_blank" href=" http://www.scirp.org/journal/ajac/"> Texto integral </a> <br>

Abstract (EN): This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, in-corporated in both PVC and sol-gel membranes and directly applied onto a conductive graphite/epoxy resin support. The units were constructed without inner reference solution adopting conventional configuration and in the case of PVC membrane the tubular configuration was also adopted. The good working character-istics of these electrodes, made possible its application to the determination of ampicillin in pharmaceuticals formulations, both in conventional batch analysis and in flow conditions, when the electrodes were coupled to a SIA system. In the last case the potentiometric sensors presented linear response towards ampicillin concentration between 5.0 × 10–4 and 5.0 × 10–2 mol·l–1 with slopes of –57.4 and –63.5 mV·dec–1 for the PVC and sol-gel membranes, respectively. The developed procedures enable mean relative standard deviations better than 3% for all the samples analysed. The obtained results do not statistically differ from those fur-nished by applying the HPLC reference method. <br> <br> Keywords: Ampicillin, SIA, Potentiometric Detection, Pharmaceutical Formulations <br> <a target="_blank" href="http://www.scirp.org/journal/ajac/ "> Full text</a> <br>

Idioma: Inglês

Tipo (Avaliação Docente): Científica