A Cyclam Salt as an Antifungal Agent: Interference with <i>Candida</i> spp. and <i>Cryptococcus neoformans</i> Mechanisms of Virulence

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

Cerqueira, F (Autor) Outra; Rui Medeiros (Autor) ICBAS; Lopes, I (Autor) Outra; Campos, C (Autor) Outra; Ferraz, MP (Autor) FEUP; Silva, F (Autor) Outra; Alves, LG (Autor) Outra; Pinto, E (Autor) Outra

Revista

Título: Antibiotics

Vol. 13

Página Final: 222

Editora: MDPI

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Classificação Científica

CORDIS: Ciências Tecnológicas ; Ciências da Saúde

FOS: Ciências médicas e da saúde ; Ciências da engenharia e tecnologias

Outras Informações

ID Authenticus: P-010-1J8

DOI: 10.3390/antibiotics13030222

Abstract (EN): The importance of fungal infections, particularly those caused by yeasts, is increasing among the medical community. Candida albicans and Cryptococcus neoformans are amongst the high-priority fungal species identified by the World Health Organization (WHO) and are considered in the critical group, while Candida krusei is included in the medium-priority group. The cyclam salt H4[H2(4-CF3PhCH2)2Cyclam]Cl4 proved to be active against the growth of these three yeasts, and the aim of this work was to verify its interference with their virulence mechanisms, whether shared or unique. H4[H2(4-CF3PhCH2)2Cyclam]Cl4 significantly inhibited biofilm production and catalase activity, being able to interfere with C. albicans dimorphic transition and C. neoformans melanin production. At the minimal inhibitory concentration (MIC) values, H4[H2(4-CF3PhCH2)2Cyclam]Cl4 had no antioxidant effect, as determined by the DPPH method. When using the RAW264.7 macrophage cell line, H4[H2(4-CF3PhCH2)2Cyclam]Cl4 reduced nitric oxide (NO) detection (the Griess reaction), but this effect was associated with a significant toxic effect on the cells.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13