NO Fluorescence Sensing by Europium Tetracyclines Complexes in the Presence of H2O2

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Eliana F C Simoes (Autor) Outra; Joao M M Leitao (Autor) Outra; Joaquim C G E Esteves da Silva (Autor) FCUP

Revista

Título: Journal of Fluorescence

Vol. 23

Páginas: 681-688

ISSN: 1053-0509

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

ISI Web of Science

Scopus - 5 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química

Outras Informações

ID Authenticus: P-006-9ME

DOI: 10.1007/s10895-013-1207-9

Abstract (EN): The effect on the fluorescence of the europium:tetracycline (Eu:Tc), europium:oxytetracycline (Eu:OxyTc) and europium:chlortetracycline (Eu:ClTc) complexes in approximately 2:1 ratio of nitric oxide (NO), peroxynitrite (ONOO-), hydrogen peroxide (H2O2) and superoxide (O-2 (center dot-)) was assessed at three ROS/RNS concentrations levels, 30 A degrees C and pH 6.00, 7.00 and 8.00. Except for the NO, an enhancement of fluorescence intensity was observed at pH 7.00 for all the europium tetracyclines complexes-the high enhancement was observed for H2O2. The quenching of the fluorescence of the Tc complexes, without and with the presence of other ROS/RNS species, provoked by NO constituted the bases for an analytical strategy for NO detection. The quantification capability was evaluated in a NO donor and in a standard solution. Good quantification results were obtained with the Eu:Tc (3:1) and Eu:OxyTc (4:1) complexes in the presence of H2O2 200 mu M with a detection limit of about 3 mu M (Eu:OxyTc).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8