Theoretical Photodynamic Study of the Photoprotolytic Cycle of Firefly Oxyluciferin

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

da silva, lp (Autor) Outra; simkovitch, r (Autor) Outra; huppert, d (Autor) Outra; da silva, jcge (Autor) FCUP

Revista

Título: ChemPhysChem

Vol. 14

Páginas: 2711-2716

ISSN: 1439-4235

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 22 Citações

ISI Web of Science

Scopus - 19 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química

Outras Informações

ID Authenticus: P-006-85F

DOI: 10.1002/cphc.201300330

Abstract (EN): Firefly oxyluciferin presents a pH-sensitive fluorescence in aqueous solutions. Its fluorescence spectra are composed of two green peaks at different pH values, despite the enolate anion being the only emitter. A computational approach was used to further elucidate the photoprotolytic cycle of oxyluciferin and investigate its pH sensitivity. It was found that oxyluciferin forms - stacking complexes both in the ground and excited states, at basic and acidic/neutral pH. However, at different pH values, these complexes adopt a different conformation, which explains the lower energy of the emission at acidic/neutral pH, in comparison with the emission at basic pH.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6