Molecularly imprinted polymer SPE sensor for analysis of CA-125 on serum

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Rebelo, TSCR (Autor) Outra; Costa, R (Autor) FCUP; Brandao, ATSC (Autor) FCUP; Silva, F (Autor) FCUP; Sales, MGE (Autor) Outra; Carlos M Pereira (Autor) FCUP

Revista

Título: Analytica Chimica Acta

Vol. 1082

Páginas: 126-135

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 51 Citações

Scopus - 67 Citações

Outras Informações

ID Authenticus: P-00R-0BR

DOI: 10.1016/j.aca.2019.07.050

Abstract (EN): Considering the high incidence level and mortality rate of ovarian cancer, particularly among the European female population, the carbohydrate antigen 125 (CA-125) was selected as the protein target for this study for the development of a MIP-based biosensor. This work presents the development of molecular imprinting polymers (MIPs) on gold electrode surface for CA-125 biomarker recognition. The preparation of the CA-125 imprinting was obtained by electropolymerization of pyrrole (Py) monomer in a gold electrode using cyclic voltammetry (CV) in order to obtain highly selective materials with great molecular recognition capability. The quantification of CA-125 biomarker was made through the comparison of two methods: electrochemical (square wave voltammetry -SWV) and optical transduction (surface plasmon resonance -SPR). SWV has been widely used in biological molecules analysis since it is a fast and sensitive technique. In turn, SPR is a non-destructive optical technique that provides high-quality analytical data of CA-125 biomarker interactions with MIP. Several analytical parameters, such as sensitivity, linear response interval, and detection limit were determined to proceed to the performance evaluation of the electrochemical and optical transduction used in the development of the CA-125 biosensor. The biosensor based in the electrochemical transduction was the one that presented the best analytical parameters, yielding a good selectivity and a detection limit (LOD) of 0.01 U/mL, providing a linear concentration range between 0.01 and 500 U/mL. This electrochemical biosensor was selected for the study and it was successfully applied in the CA-125 analysis in artificial serum samples with recovery rates ranging from 91 to 105% with an average relative error of 5.8%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10