Separation Processes in Biotechnology

Code:

M.BIO036

Acronym:

PSBI

Keywords
Classification	Keyword
OFICIAL	Biological Engineering

Instance: 2024/2025 - 1S

Active?	Yes
Responsible unit:	Department of Chemical and Biological Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M.BIO	20	Syllabus	1	-	6	52	162
M.EQ	20	Syllabus	1	-	6	52	162

Teaching language

Portuguese
Obs.: Português - Suitable for English-speaking students

Objectives

To acquire advanced engineering knowledge in purification processes of biological products, as well as their approval and regulation. To develop students’ reasoning skills, as well as their capacity to analyse production diagrams and to use bioinformatic tools.

Learning outcomes and competences

Learn how to analyze an industrial production process particularly the operation units concerning downstream processing. Learn how to select the most adequate equipment for each operation unit and to perform equipment sizing in particular cases.

Working method

Presencial

Program

1- Biotechnology and bioproducts 2- Introduction to the purification of biological products 3- Cell rupture a) Mechanical methods b) Non-mechanical methods 4- Solid-liquid separation a) Sedimentation b) Centrifugation c) Conventional filtration d) Membrane processes 5- Chromatography a) Gel filtration b) Ionic exchange c) Affinity d) Hydrophobic interaction 6- Extraction processes 7- Precipitation and crystallization 8- Solvent removal a) Drying b) Freeze drying c) Evaporation 9- Product activity 10- Regulation a) Validation b) Approval c) Formulation 11 - Analysis of production diagrams 12- Simulation of purification processes

Mandatory literature

Harrison, Roger G. 070; Bioseparations science and engineering. ISBN: 0-19-512340-9
Nuno F. Azevedo; Diapositivos de apoio à UC. ISBN: (A fornecer aos estudantes em formato eletrónico)

Complementary Bibliography

Crommelin, Daan J. A. 340; Pharmaceutical biotechnology. ISBN: 0-415-28501-1
Wink, M.; An introduction to molecular biotechnology, Wiley-VCH, 2006. ISBN: 3-527-31412-1

Teaching methods and learning activities

Oral presentation with the support of the board and slides. 

keywords

Natural sciences > Biological sciences > Biological engineering
Technological sciences > Engineering

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Designation	Weight (%)
Trabalho escrito	40,00
Exame	60,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Estudo autónomo	24,00
Frequência das aulas	56,00
Trabalho escrito	80,00
Total:	160,00

Eligibility for exams

N/A

Calculation formula of final grade

Final grade will be obtained by an weighted average of the several components of the final work, the classification from the oral presentation and the report (corresponding to 40% of the final grade). The exam grade corresponds to 60% of the final grade

Examinations or Special Assignments

Students will have to do a group assignment, being the assignment sheet delivered at the beginning of the semester. The assignment is to be carried out during the semester (some parts will be carried out in class). The group assignment comprises: a) Introduction b) Patent research c) Development of a production diagram d) Simulation of purification processes e) Conclusion. This work will be orally presented and discussed in class.

Special assessment (TE, DA, ...)

By globla exam on the resit period. Admittance to that exam requires the delivery of the group work with all its components and with a final evaluation of at least 7/20 points.

Classification improvement

Students can improve their grades by attending an exam at the recurso (resit) season.