Integrated Project in Biological Engineering II

Code:

EBE0135

Acronym:

PIEB2

Keywords
Classification	Keyword
OFICIAL	Biological Engineering

Instance: 2012/2013 - 1S

Active?	Yes
Responsible unit:	Department of Chemical Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIB	16	Syllabus	4	-	6	56	162
MIEQ	11	Syllabus	5	-	6	56	162

Teaching language

Suitable for English-speaking students

Objectives

The main objective of the Integrated Project in Biological Engineering II (4 th year of the MSc in Bioengineering - Biological Engineering, 5th year of the MSc in Chemical Engineering - Biotechnology) is to provide laboratorial support to the courses of Chemistry and Technology of Products, Separation Processes in Biotechnology and Environmental Technology. As this is a laboratory course, the assessment is made primarily from the reports made. The performance in class will be also an important element of evaluation. The students will execute six works and will prepare a report for each work proposed (5 reports as group report and one individual).
At the end of the course, the students should be able to operate and interpret the data resulting from the application of a wide range of methods with potential use in diverse industries (chemical, biotech, pharmaceutical, food,sewage treatment, etc.).

Program

1. Physical and chemical analysis of a synthetic wastewater

2. Treatment of a synthetic wastewater - comparison of alternative strategies: i) complete mix activated sludge ii) rotating biodisc system

3. Drying of apple

4. Determination of acidity and peroxide values of oils

5. Production and purification of a GFP

6. Simulation of operating an anaerobic reactor for the treatment of dewatered sludge from WWTP

7. Proposal and evaluation of alternatives to the practical work performed.

Mandatory literature

APHA, AWWA, WPCF. ; Standard methods for the examination of water and wastewater. 17ª edição, Clesceri LS, Greenberg AE, Trussel RR. (eds.), Washington DC, USA., 1987
Henze M, Harremöes P, Jansen JLC, Arvin E.; Wastewater treatment: biological and chemical processes., Springer-Verlag., 1995
Mariotti E, Mascini M.; Determination of extra virgin olive oil acidity by FIA-titration., Food Chemistry. Elsevier, 2001
Takechi T, Takamura H, Matoba T.; Application of colorimetric method for determination of lipid peroxides in food., Food Science and Technology Research. Elsevier, 2004
Mergulhão F, Monteiro G.; Analysis of factors affecting the periplasmic production of recombinant proteins on Escherichia coli., Journal of Microbiology and Biotechnology. - Korean Society for Microbiology and Biotechnology, 2007
de Azevedo EG, Alves AM. ; Engenharia de Processos de Separação, Instituto Superior Técnico, 2010
de Azevedo EG, Alves AM. ; Engenharia de Processos de Separação, Instituto Superior Técnico, 2010

Complementary Bibliography

Metcalf & Eddy, Inc. revised by Tchobanoglous G, Burton FL.; Wastewater Engineering – treatment, disposal, and reuse., McGraw-Hill, Inc., 1991
Diário da República.; Decreto Lei nº 236/98, de 1 de Agosto. I série A., 1998
Harrison RG, Todd P, Rudge SR, Petrides DP.; Bioseparations science and engineering. , Oxford University Press., 2003
Structural Genomics Consortium et al.; Protein production and purification. , Nature Methods. Nature Publishing Group, 2008

Teaching methods and learning activities

Formal presentation of some theoretical and experimental methodologies. It is proposed that students perform independently, with supervision and with theoretical support, the proposed laboratorial work.

Software

Matlab
Microsoft windows
Adobe Acrobat

keywords

Technological sciences > Engineering
Natural sciences > Biological sciences > Biological engineering

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
Attendance (estimated)	Participação presencial	52,00
	Total:	-	0,00

Calculation formula of final grade

The final grade will be calculated according to the following components:
Group reports with a weighting factor of 60% in the final classification (the overall report will have a weighting factor of 25%. The remaining work will have a weighting factor of 35%);
Individual report of an experimental study with a weighting factor of 20% in the final classification;
Oral presentation of an experimental study with a weighting factor of 5% in the final classification;
Continuous assessment - performance in class with a weighting factor of 15% in the final classification.

To obtain positive rating, the student should not exceed the limit of absences allowed by law and must obtain a minimum overall rating of 9.5.
The act of presenting a report containing part of a work that belongs to someone else, without putting the credits to the original author, will result in a null classification for that evaluation component.

Special assessment (TE, DA, ...)

These situations do not preclude the completion of the works proposed. These have to be performed at the same time limit established for regular students. The form of assessment is therefore the same for all students.

Classification improvement

Laboratorial examination and presentation (writing and oral) of the work (to be improved) developed.