Separation Processes in Biotecnology

Code:

EBE0133

Acronym:

PSBI

Keywords
Classification	Keyword
OFICIAL	Biological Engineering

Instance: 2012/2013 - 1S

Active?	Yes
Responsible unit:	Department of Chemical and Biological Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIB	16	Syllabus	4	-	6	56	162
MIEQ	10	Syllabus	4	-	6	56	162
			5

Teaching language

Portuguese

Objectives

To acquire advanced engineering knowledge in purification processes of biological products, as well as their approval and regulation.
To develop students’ reasoning skills, as well as their capacity to analyse production diagrams and to use bioinformatic tools.

Program

1- Biotechnology and bioproducts

2- Introduction to the purification of biological products

3- Cell rupture
a) Mechanical methods
b) Non-mechanical methods


4- Solid-liquid separation
a) Sedimentation
b) Centrifugation
c) Conventional filtration
d) Membrane processes

5- Chromatography
a) Gel filtration
b) Ionic exchange
c) Affinity
d) Hydrophobic interaction

6- Extraction processes

7- Precipitation and crystallization

8- Solvent removal
a) Drying
b) Freeze drying
c) Evaporation

9- Product activity

10- Regulation
a) Validation
b) Approval
c) Formulation

11-Bioinformatic analysis of proteins

12- Analysis of production diagrams

13- Simulation of purification processes

Mandatory literature

Harrison, Roger G. 070; Bioseparations science and engineering. ISBN: 0-19-512340-9
Filipe Mergulhão; Textos de apoio da disciplina, 2009. ISBN: (A fornecer aos alunos em formato electrónico)

Complementary Bibliography

Crommelin, Daan J. A. 340; Pharmaceutical biotechnology. ISBN: 0-415-28501-1
Wink, M.; An introduction to molecular biotechnology, Wiley-VCH, 2006. ISBN: 3-527-31412-1

Teaching methods and learning activities

Oral presentation with the support of the board and slides. Classes at the computer room using simulation software and bioinformatic tools available on the web.

keywords

Natural sciences > Biological sciences > Biological engineering
Technological sciences > Engineering

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
Attendance (estimated)	Participação presencial	52,00
	Total:	-	0,00

Eligibility for exams

According to General Evaluation Rules of FEUP, students have to attend classes.

Calculation formula of final grade

Final Grade will be based on the following formula:

FG= 0.6x A + 0.3xOP + 0.1D

A- grade of the group assignment
OP- Oral presentation and discussion of the assignment
D- Presentation and discussion of the production diagram

To complete this course unit, students have to reach a minimum grade of 8 in the above mentioned assessment component. Besides, they have to reach a minimum average grade of 10.

Examinations or Special Assignments

Students will have to do a group assignment, being the assignment sheet delivered at the beginning of the semester. The assignment is to be carried out during the semester (some parts will be carried out in class).

The group assignment comprises:

a) Introduction
b) Patent research
c) Development of a production diagram
d) Bioinformatic analysis of proteins
e) Simulation of purification processes
f) Conclusion

Special assessment (TE, DA, ...)

It will take place an exam at the special season of exams, which will substitute the grade of the presentations (according to General Evaluation Rules of FEUP).

Classification improvement

Students can improve their grades by attending an exam at the recurso (resit) season.