Enzimatic Engineering

Code:

EBE0189

Acronym:

EENZ

Keywords
Classification	Keyword
OFICIAL	Biological Engineering
OFICIAL	Bioprocess Engineering

Instance: 2012/2013 - 2S

Active?	Yes
Responsible unit:	Department of Chemical and Biological Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIB	21	Syllabus	3	-	6	56	162
MIEQ	24	Syllabus	4	-	6	56	162

Teaching language

Suitable for English-speaking students

Objectives

It is intended that students understand the essence of biocatalytic processes; are able of modeling enzyme kinetics; acquire the ability to simulate the operation of enzymebioreactors.

Another objective is to give students basic skills to design, measure and optimize the operation of enzymatic reactors in various systems, and consequently enhance their proficiency in the development and implementation of biotechnology-based industrial processes incorporating enzyme technology.

 

Learning outcomes and competences

By completing this course the student should be able to:

• Understand the role of enzymes in biotechnological applications

• Document the major technological applications of enzymes

• Know the properties of enzymes and their mode of operation

• Identify the types of mechanisms of enzymatic catalysis

• Describe the process of biocatalysis in non-conventional media

• Modeling the enzyme kinetics

• Assess the kinetic model of the reaction mechanism

• Describe the usefulness of enzyme biosensors

• Know the types of enzyme inhibition

• Describe the types of enzyme immobilization

• Calculate the effects of immobilization on enzyme kinetics

• Know the main types of enzyme reactors

• Modeling the main types of enzymatic reactors

 

Working method

Presencial

Program

1. General properties of enzymes. General characteristics of the amino acids. Classification and nomenclature of enzymes. Levels of enzyme structure. The structure/function in an enzyme. Specificity of enzymes. Main types of mechanisms for enzyme catalysis.

 

Biocatalysis in aqueous medium. Biocatalysis in organic solvents, ionic liquids, fluids supercrítricos systems, solid-solid and solid-liquid systems. Water effects on the enzyme activity and stability. 

 

3. Enzyme technologies. Major industrial applications of enzymes. Enzymatic biosensors and clinical diagnostics. 

 

4. Enzyme kinetics and stability. Michaelis-Menten model. Complementary and alternative models. Assessment of the kinetic constants. Enzyme activity and stability. Effect of pH and temperature on enzyme kinetics. Types of chemical inhibition.

 

5. Immobilization of enzymes. Types of supports and immobilization techniques (adsorption, ionic binding, covalent binding, cross-linking, microencapsulation and occlusion). Effects of enzyme and kinetic properties (conformational, partition and mass transfer). External and internal diffusional limitations. Efficiency factor. 

 

6. Enzymatic Reactors: Classification. Main types of ideal reactors and their equations. Real (non-ideal) reactor: residence time distribution and degree of mixing.

 

Mandatory literature

James M. Lee; Biochemical engineering. ISBN: 0-13-085317-8
Andreas S. Bommarius, Bettina R. Riebel; Biocatalysis. ISBN: 978-3-527-30344-1

Complementary Bibliography

James E. Bailey, David F. Ollis; Biochemical engineering fundamentals. ISBN: 0-07-066601-6
Cabral J.M.S., Aires-Barros M.R., Gama M. ; Engenharia Enzimática. , Lidel – edições técnicas, Lda., 2003

Teaching methods and learning activities

Classes will be based on the formal presentation of the theoretical concepts and methodologies to approach and solve problems, along with examples of application. Students will be asked to read and present some relevant scientific papers. It will also be proposed examples of dimensioning of enzymatic bioreactors, where the fundamental rules of modelling and design are applied.

Software

Acrobat Professional 9.0 Win AOO License IE
Microsoft

keywords

Natural sciences > Biological sciences > Biological engineering

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
Attendance (estimated)	Participação presencial	68,00	10,00
	Exame	3,00	60,00
	Trabalho escrito	8,00	30,00
	Total:	-	100,00

Amount of time allocated to each course unit

Description	Type	Time (hours)	End date
	Estudo autónomo	73
Tutorial	Estudo autónomo	10
	Total:	83,00

Calculation formula of final grade

The final grade will be based on the following assessment components:
Final exam (60%): questions for development on the points 1,2 and 3, multiple choice questions on points 4, 5 and 6.
Critical analysis of a topic in enzymatic engineering (15%).
Presentation and discussion of a scientific paper (15%).
Interest and participation in the classes (10%).
To obtain positive rating, the student must have an overall grade of at least 9.5 in 20. However, the final exam must have a score higher than 8 in 20.

 

Special assessment (TE, DA, ...)

These students may be exempted from continuous assessment.

Classification improvement

Only the final exam. For regular students, the grade of the work is valid for the entire school year, and can not be replaced by another type of evaluation.