Integrated Laboratories I

Code:

EBE0111

Acronym:

LI1

Keywords
Classification	Keyword
OFICIAL	Basic Sciences

Instance: 2012/2013 - 1S

Active?	Yes
Responsible unit:	Department of Chemical and Biological Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIB	69	Syllabus	1	-	4	70	108

Teaching language

Portuguese

Objectives

This course unit aims to familiarise students with knowledge in fundamental sciences related to the program of the course units of Fundamentals of Chemistry, Fundamentals of Physics/Biophysics and Cellular and Molecular Biology, by performing experiments.
This course unit also aims to develop students’ skills in: bibliography research, written communication, scientific reasoning, critical thinking regarding results obtained and teamwork.

Program

The first class will cover the concepts of laboratory safety and laboratory waste management. The following classes will be based on laboratory assignments according to the areas of Chemistry, Biology and Physics:
A1- Standard substances and standardization; Notions of error and uncertainty;
A2- Quantification of chloride anion in saliva (interstitial fluids)
A3- Equipment calibration (automatic burette);
A4- Ionic and covalent bonding; Solution conductivity of ionic and covalent compounds;
A5- Behaviour of acids and bases in aqueous solution: buffer capacity of solutions used in biological systems;
A6- Recycling aluminium drinks cans;
A7- Study of enzymatic reaction kinetics: calibration curve and enzymatic test;
A8- Cellular diversity;
A9- Epithelial tissue;
A10 and 11- Connective tissue;
A12- Determination of the velocity of production of lactic acid by yogurt microorganisms;
A13- Imunolabeling of cytokeratin;
A14- Production of silver chloride electrodes to monitor biological signals; to obtain an electrocardiographic register;
A15a-b - Crystallization of proteins;
A16a-b - Cellular fractionation; Study of sedimentation in preparative centrifugation.


At the end of each assignment, students have to do the necessary calculation to present the results. Besides they have to write a summary report (max of 2 pages).

Mandatory literature

Bergethon P R; The Physical Basis of Biochemistry, Springer-Verlag, 1998
Wilson, K & Walker, J.; Principles and Techniques of Practical Biochemistry, Cambridge University Press, 2000
Junqueira, Luiz C. & carneiro, José; Histologia Básica - texto e atlas, Guanabara Koogan, 2008
Skoog, Douglas A.; Fundamentals of Analytical Chemistry. ISBN: 0-03-074922-0
Vogel, A.I.; Análise Química Quantitativa, 6ª Ed., 2002. ISBN: Livros Técnicos e Científicos

Teaching methods and learning activities

The above mentioned assignments will be carried out during practical classes.
Students have to take notes about the theoretical principles, procedures, safety rules and calculations used. Students have to take their notebooks to all practical classes, because they have to take notes about the alteration of procedures, observation and to register data (schemes, tables and graphics). At the end of each assignment, students have to do the necessary calculation to present the results.
All notes will be the base to write a summary report (max. of 2 pages*).

*The results of the assignments, which encompass more than one class, have to be written in only one report. The number of pages is proportional to the number of classes.

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
Attendance (estimated)	Participação presencial	65,00
	Total:	-	0,00

Eligibility for exams

Laboratory classes are mandatory to all students, even to the ones in accordance with the article 4, lines a) and b) of General Evaluation Rules of FEUP.

Students cannot miss more classes than allowed by the rules. Furthermore they have to reach a minimum grade of 10.

Calculation formula of final grade

The Final Grade of this course unit will cover 3 different components:
1. Assessment of each laboratory assignment (LAi). At the end of each laboratory assignment, each group of students has to deliver a summary report (max of 2 pages), which should be included in the notebook.
This component will assess students’ performance at the laboratory: assiduity, preparation of the assignments and practical performance.
LA= total LAi/n laboratory assignments

2. Assessment of the laboratory notebook. Students should keep their notebooks updated.
At the end of the semester, they should write on the notebook an index. It will be assessed the register related to two laboratory assignments of the same topic (Biology, Chemistry or Physics- randomly chosen). It will be taken into account the level of preparation, data register and important notes which are necessary to properly carry out and discuss an experimental assignment.

The notebook (NB) evaluation is given by:

CC = 0,3evaluation stage1 + 0,7evaluation stage2
(3 lab experiments will be evaluated during evaluation stage1 and 4 during stage2)

The collection of the lab notebook after its assessment at different evaluation stages implies the acceptanceof the grade by the student.


Report contribution (CR) of chemistry and biology (CRQ /CRB) is calculated as:

CRQ/B = 0,4 stage1 + 0,6stage2

The contribution of Physics (CRF) is given by:

CRF=average of all reports/notes evaluated in Physics

The practical component is then calculated as:

PC= 0,35CC + 0,6[(0,4(CRQ) + 0,4(CRB) + 0,2(CRF)]

3. Test (T)- final exam at the end of the semester, after all the reports and notebooks have been delivered and corrected. It will include questions related to the laboratory assignments.

Final Grade:
FG= 0.5xPC + 0.5xT

Examinations or Special Assignments

Not applicable

Special assessment (TE, DA, ...)

Not applicable

Classification improvement

Students with T <7 or who want to improve their grades, can attend a second written exam, which will take place at recurso (resit) season.