Evaluation of GSH adducts of adrenaline in biological samples

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Renata Silva (Author) Other; Silvia Boldt (Author) Other; Vera Marisa Costa (Author) Other; Helena Carmo (Author) FFUP; Marcia Carvalho (Author) Other; Felix Carvalho (Author) FFUP; Maria de Lourdes Bastos (Author) FFUP; Francisco Lemos Amado (Author) Other; Fernando Remiao (Author) FFUP

Journal

Title: Biomedical Chromatography

Vol. 21 No. 1

Pages: 670-679

ISSN: 0269-3879

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 12 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-8YW

DOI: 10.1002/bmc.796

Abstract (EN): The sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity. Copyright (c) 2007 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10