Fusion with fluorescent proteins for subcellular localization of enzymes involved in plant alkaloid biosynthesis

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Duarte, P (Author) Other; Memelink, J (Author) Other; Sottomayor, M (Author) FCUP

Journal

Title: Methods in molecular biology (Clifton, N.J.)

Vol. 49

Pages: 275-290

ISSN: 1064-3745

Publisher: Springer Nature

Indexing

ISI Web of Knowledge

Scopus - 5 Citations

Other information

Authenticus ID: P-00F-WM0

DOI: 10.1007/978-1-60761-723-5_19

Abstract (EN): To establish the role in alkaloid metabolism of candidate genes identified in silico or by Omics approaches, it may be essential to determine the subcellular localization of the encoded proteins. The fusion with fluorescent proteins (FP) may now be used as a quite effective and reliable tool to investigate this question. The methodology involves the choice of the FP, the design and production of the appropriate FP fusions, and the use of a transient or stable transformation protocol applied to a homologous or heterologous plant system. This chapter describes the application of this methodology to an enzyme involved in indole alkaloid biosynthesis, with general considerations on the development of the approach. © 2010 Springer Science+Business Media, LLC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16