4 '-Methoxy-2-styrylchromone a novel microtubule-stabilizing antimitotic agent

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Joana Marinho (Author) Other; Madalena Pedro (Author) Other; Diana C G A Pinto (Author) Other; Artur M S Silva (Author) Other; Jose A S Cavaleiro (Author) Other; Claudio E Sunkel (Author) ICBAS; Maria Sao Jose Nascimento (Author) FFUP

Journal

Title: Biochemical Pharmacology

Vol. 75 No. 4

Pages: 826-835

ISSN: 0006-2952

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 39 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-004-1G5

DOI: 10.1016/j.bcp.2007.10.014

Abstract (EN): 4 '-Methoxy-2-styrylchromone, a new synthetic chromone was identified as a selective proliferation inhibitor of human tumor (MCF-7 and NCI-H460) cell lines than to non-tumor cells (MRC-5). The antiproliferative activity of this chromone was also extensive to peripheral human lymphocytes. 4 '-Methoxy-2-styrylchromone was found to block tumor cells in the G(2)/M phase of the cell cycle. The G(2)/M arrest of NCI-H460 cells was dose- and time-dependent, reaching a maximum after 12-h treatment while MCF-7 cells reached the maximum value of G2/M accumulation only after a 24-h treatment. Chromone-treated cells evidenced a high frequency of cells in prometaphase, indicating progression beyond G(2) and arrest early in mitosis. This mitotic arrest was associated with abnormal mitotic spindles characterized by the formation of a monopolar structure, suggesting that the chromone interferes with microtubules. The results of an in vitro tubulin polymerization assay showed that this chromone stabilizes microtubules in a manner similar to paclitaxel.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10