Capillary electrophoresis analysis of a 9-plex str assay for canine genotyping

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Van Asch, B (Author) Other; Amorim, A (Author) FCUP

Journal

Title: Methods in molecular biology (Clifton, N.J.)

Vol. 11

Pages: 231-240

ISSN: 1064-3745

Publisher: Springer Nature

Indexing

ISI Web of Knowledge

Scopus - 2 Citations

Other information

Authenticus ID: P-005-49X

DOI: 10.1007/978-1-61779-461-2_16

Abstract (EN): STR analysis of canine-derived biological evidence for the identification of individuals is becoming an important tool for forensic investigations. A protocol for the multiplex PCR amplification and capillary electrophoresis of nine autosomal STRs and two fixed-size markers for sex identification in dogs and wolves is described here. The selection of the loci included in the multiplex complies with the recommendations of the International Society for Forensic Genetics in regard to human DNA analysis. The protocol is optimized for automatic fragment size detection in an ABI platform. © 2012 Springer Science+Business Media, LLC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10