In vitro hydroxyapatite adsorbed salivary proteins

• Type: Article in International Scientific Journal
• Date: 2004

Authors

vitorino, r (Author) Other; lobo, mjc (Author) Other; duarte, j (Author) FADEUP; ferrer-correia, aj (Author) Other; tomer, kb (Author) Other; dubin, jr (Author) Other; domingues, pm (Author) Other; amado, fml (Author) Other

Journal

Title: Biochemical and Biophysical Research Communications

Vol. 320

Pages: 342-346

ISSN: 0006-291X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 32 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-9DA

DOI: 10.1016/j.bbrc.2004.05.169

Abstract (EN): in spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5