In Vitro Susceptibility of Prototheca to pH and Salt Concentration

• Type: Article in International Scientific Journal
• Date: 2010

Authors

marques, s (Author) Other; silva, e (Author) Other; carvalheira, j (Author) ICBAS; thompson, g (Author) ICBAS

Journal

Title: Mycopathologia

Vol. 169

Pages: 297-302

ISSN: 0301-486X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-003-81Y

DOI: 10.1007/s11046-009-9254-0

Abstract (EN): Prototheca sp. can assume high economic significance in the dairy industry and pose a potential risk for the public health. We investigated the in vitro susceptibility of Prototheca isolates retrieved from mastitic milk (P. zopfii and P. blaschkeae) to different pH buffers and salt concentrations using a microbroth assay adapted from the Clinical Laboratory Standards Institute guidelines. Different pH buffer solutions ranging from pH 1 to pH 12 and different sodium chloride concentrations, 4.5, 9 and 18%, were tested. P. zopfii strains presented an optimal growth between pH 5 and 9, a complete growth inhibition at pH 3, and limited growth at pH 1 and 12, whereas P. blaschkeae strains showed higher susceptibility to all pH values except for pH 3 where it demonstrated a moderate growth when compared to P. zopfii strains. When salinity was incremented, P. blaschkeae was more resistant than P. zopfii, although a reduction in growth for all strains of Prototheca was observed. This study demonstrated differences in the in vitro susceptibilities of P. zopfii and P. blaschkeae to different pH and salt concentrations and intend to be a contribution on the understanding of some of the physiologic features that can be associated with the survival of these microalgae in the environment.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6