Effect of abietane diterpenes from Plectranthus grandidentatus on T- and B-lymphocyte proliferation

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Cerqueira, F (Author) Other; Cordeiro Da Silva, A (Author) FFUP; Gaspar Marques, C (Author) Other; Simoes, F (Author) Other; Pinto, MMM (Author) FFUP; Nascimento, MSJ (Author) FFUP

Journal

Title: Bioorganic and Medicinal Chemistry

Vol. 12

Pages: 217-223

ISSN: 0968-0896

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 44 Citations

Scopus - 52 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-BZT

DOI: 10.1016/j.bmc.2003.10.006

Abstract (EN): Five known abietane diterpenes of the royleanone and coleon type, namely, fatty acid esters of 7alpha-acyloxy-6beta-hydroxyroyleanone (1), grandidone A (2), 7alpha-acetoxy-6beta-hydroxyroyleanone (3), 6beta,7alpha-dihydroxyroyleanone (4) and coleon U (5), isolated from Plectranthus grandidentatus, were evaluated for their effect on the proliferation of human lymphocytes induced by the mitogen PHA. All except 4, showed a dose-dependent suppressor effect, with 3 yielding the most potent antiproliferative activity, followed by 5. These two compounds, that represent diterpenes of the royleanone and coleon type respectively, were also shown to be potent inhibitors of mouse splenocyte proliferation induced by ConA or LPS mitogens. However, the sensitivity of ConA-stimulated splenocytes to their suppressive effect was higher, suggesting a preferential inhibition of T-lymphocyte proliferation. The antiproliferative activity of 3 seemed to be exerted without affecting the expression of the lymphocyte activation marker CD69. On the contrary, 5 was shown to reduce the expression of CD69 of TCD8(+) and B-cells, suggesting a relationship between its antiproliferative effect and the expression of this early marker of activation on these cell populations. The capacity of 5 to induce apoptosis on ConA-stimulated splenocytes could also be related with its antiproliferative activity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7