The neurotoxicity of hallucinogenic amphetamines in primary cultures of hippocampal neurons

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Joao Paulo Capela (Author) Other; Silvana da Costa Araujo (Author) Other; Vera Marisa Costa (Author) Other; Karsten Ruscher (Author) Other; Eduarda Fernandes (Author) FFUP; Maria de Lourdes Bastos (Author) FFUP; Ulrich Dirnagl (Author) Other; Andreas Meisel (Author) Other; Felix Carvalho (Author) FFUP

Journal

Title: NeuroToxicology

Vol. 34

Pages: 254-263

ISSN: 0161-813X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 29 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-1MD

DOI: 10.1016/j.neuro.2012.09.005

Abstract (EN): 3,4-Methylenedioxymethamphetamine (MDMA or "Ecstasy") and 2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) are hallucinogenic amphetamines with addictive properties. The hippocampus is involved in learning and memory and seems particularly vulnerable to amphetamine's neurotoxicity. We evaluated the neurotoxicity of DOI and MDMA in primary neuronal cultures of hippocampus obtained from Wistar rat embryos (E-17 to E-19). Mature neurons after 10 days in culture were exposed for 24 or 48 h either to MDMA (100-800 mu M) or DOI (10-100 mu M). Both the lactate dehydrogenase (LDH) release and the tetrazolium-based (MTT) assays revealed a concentration- and time-dependent neuronal death and mitochondrial dysfunction after exposure to both drugs. Both drugs promoted a significant increase in caspase-8 and caspase-3 activities. At concentrations that produced similar levels of neuronal death, DOI promoted a higher increase in the activity of both caspases than MDMA. In the mitochondrial fraction of neurons exposed 24 h to DOI or MDMA, we found a significant increase in the 67 kDa band of apoptosis inducing factor (AIF) by Western blot. Moreover, 24 h exposure to DOI promoted an increase in cytochrome c in the cytoplasmatic fraction of neurons. Pre-treatment with an antibody raised against the 5-HT2A-receptor (an irreversible antagonist) greatly attenuated neuronal death promoted by 48 h exposure to DOI or MDMA. In conclusion, hallucinogenic amphetamines promoted programmed neuronal death involving both the mitochondria machinery and the extrinsic cell death key regulators. Death was dependent, at least in part, on the stimulation of the 5-HT2A-receptors.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10