An automatic Method to identify and extract information of DNA bands in Gel Electrophoresis Images

• Type: Article in International Conference Proceedings Book
• Date: 2009

Authors

Caridade, CMR (Author) Other; Marcal, ARS (Author) FCUP; Mendonca, T (Author) FCUP; Pessoa, AM (Author) Other; Pereira, S (Author) FCUP

Conference proceedings International

Title: 2009 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-20

Pages: 1024-1027

Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society

Minneapolis, MN, SEP 03-06, 2009

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 2 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-003-PRW

DOI: 10.1109/iembs.2009.5332462

Abstract (EN): This paper presents a system for the automatic processing of Digital Images obtained from Gel Electrophoresis. The system identifies automatically the number and the location of lanes in the digital image, as well as the location of bands on each lane, without any intervention from the user. A reference lane with a know substance is used to compute the molecular weight of the observed (unknown) bands. The system performance was tested using 12 images, obtained from 4 gels with 3 different exposures. A total of 5443 bands were tested in 12 images, 672 reference / observed lane pairs. The average error in the estimation of molecular weight of 9.2%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4