Membrane structure and interactions of a short Lycotoxin I anaiogue

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Adao, R (Author) Other; Seixas, R (Author) Other; Gomes, P (Author) FCUP; Costa C Pessoa (Author) Other; Bastos, M (Author) FCUP

Journal

Title: Journal of Peptide Science

Vol. 14

Pages: 528-534

ISSN: 1075-2617

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 16 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-0M0

DOI: 10.1002/psc.993

Abstract (EN): Lycotoxin I and Lycotoxin II are natural anti-microbial peptides, that were identified in the venom of the Wolf Spider Lycosa carolinensis. These peptides were found to be potent growth inhibitors for bacteria (Escherichia coli) and yeast (Candida glabrata) at micromolar concentrations. Recently, shortened analogues of Lycol and LycoII have been reported to have decreased haemolytic effects. A shorter Lyco-I analogue studied, Lycol 1-15 (H-IWLTALKFLGKHAAK-NH2), was active only above 10 pm, but was also the least haemolytic. On the basis of these findings, we became interested in obtaining a deeper insight into the membrane activity of LycoI 1-15, as this peptide may represent the first major step for the future development of selective, i.e. non-haemolytic, Lycotoxin-based antibiotics. The interaction of this peptide with liposomes of different composition was studied by microcalorimetry [differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC)l and CD. The results obtained from the calorimetric and spectroscopic techniques were jointly discussed in an attempt to further understand the interaction of this peptide with model membranes. Copyright (c) 2007 European Peptide Society and John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific